Figure 4. Example calculation of the specific activity of Lcp_K30.

In this experiment an assay volume of 1 ml was used. The values for time (X) and oxygen saturation (Y) are measured as described and extracted from the resulting *.csv file into a spreadsheet. The data was visualized in an X-Y scatterplot. The addition of enzyme after 12 min resulted in a decrease of the oxygen saturation due to incorporation of dioxygen during cleavage of polyisoprene. The slope can be fitted and is proportional to the enzyme activity. By multiplying the proportionality factor and dividing by the enzyme quantity used in the measurement, the specific enzyme activity can be calculated as described.