| Minute | Water (%) | Methanol (%) | |
|---|---|---|---|
| 0-5 | 50 | 50 | isocratic |
| 5-25 | 0 | 100 | gradient |
| 25-35 | 0 | 100 | isocratic |
| 35-36 | 50 | 50 | gradient |
| 36-40 | 50 | 50 | isocratic |
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