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. 2017 Feb 5;7(3):e2120. doi: 10.21769/BioProtoc.2120

Table 1. Data analysis step by step.

The table reports the data analysed in a worksheet of Excel program (Microsoft Excel). All data processed are in bold. The means of radioactivity values normalized to 1 μg of DNA are in red. The DNA content in 0.1 ml of nuclei suspension was calculated as indicated in Nuclear DNA extraction and quantification paragraph.

HCT 116 LoVo
μg DNA/0.1 ml nuclei 2.60 3.50
Blank 1 dpm 1,821.00 1,347.70
Blank 2 dpm 1,691.90 1,058.90
Blank mean (Bm) 1,756.45 1,203.30
Replicate 1 dpm 86,746.30 53,976.10
Replicate 2 dpm 78,799.60 56,090.10
Replicate 3 dpm 86,667.70 52,702.90
Replicate 1 dpm - Bm 84,989.85 52,772.80
Replicate 2 dpm - Bm 77,043.15 54,886.80
Table 1. Continued
Replicate 3 dpm - Bm 84,911.25 51,499.60
(Repl.1 dpm - Bm)/μg DNA 32,688.40 15,077.94
(Repl.2 dpm - Bm)/μg DNA 29,631.98 15,681.94
(Repl.3 dpm - Bm)/μg DNA 32,658.17 14,714.17
(Repl. dpm - Bm)/μg DNA mean 31,659.52 15,158.02
standard deviation 1,755.96 488.83