Figure 3. SEC separation profiles of lysates prepared from untreated, Cycloheximide (CHX)-treated or Emetine-treated HeLa cells.

A) Lysates prepared from untreated HeLa cells (blue line) or cells treated with CHX (red line) or Emetine (green) were analyzed by Ribo Mega-SEC using a 2,000 Å SEC column with a flow rate of 0.8 ml/min. B) Quantitation of the area under the curve in the polysome region shown in Figure 1A. Dots indicate biological replicates. *P < 0.05, **P < 0.01.