Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jul 3;40(33):5168–5181. doi: 10.1038/s41388-021-01916-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 7 — A Immunoblotting of FTO and HIF-1α levels in DLD1 cells after exposure to hypoxic conditions at several time points (0, 6, 12, 24, 48 h) (left). Relative FTO protein levels (versus Vinculin) (right). B Relative FTO mRNA level in DLD1 and HCT116 cells after exposure to hypoxic conditions at different time points. C Representative fluorescence overlay images of FTO and HIF-1α expression in mouse HCT116 induced subcutaneous tumor tissue. The yellow color in the merged images indicates the colocalization of FTO and HIF-1α. Scale bar: 200 μm D Immunoblotting of FTO levels in DLD1 and HCT116 cells followed by treatment with CHX (100 μg/mL) for the indicated times (0, 3, 6, 9, 12 h). E Immunoblotting of FTO levels in DLD1 and HCT116 cells cultured under normoxia or hypoxia for 24 h and treated with DMSO or 10 μM MG132 for 12 h. F Representative immunoblotting of ubiquitinated FTO from the IP assay of DLD1 and HCT116 cells which were co-transfected with HA-tagged Ub plasmid and Flag-tagged FTO plasmid, then exposed to normoxic (N) and hypoxic (H) conditions for 24 h. G The FTO (STRAP) proteins were immunoprecipitated with STRAP (FTO) antibody in DLD1 and HCT116 cells, while IgG pulldown was used for IP control. The immunoblots were probed with FTO and STRAP antibodies to test the interaction between FTO and STRAP. H Immunoblotting (left) and relative FTO and STRAP protein levels (right) after STRAP knockdown in DLD1 cells exposed to 21% oxygen and 1% oxygen for 24 h. I The immunoblotting of the ubiquitination assay from DLD1 and HCT116 cells after STRAP knockdown and co-transfected with HA-tagged Ub plasmid and Flag-tagged FTO plasmid. J Representative immunoblotting of the ubiquitination assay from wild type (WT) or mutant DLD1 cells which were co-transfected with HA-tagged Ub plasmid and Flag-tagged FTO plasmid under hypoxia for 24 h. Data in (A, B, and H) are presented as the means ± S.D. (n = 3) *P < 0.05, **P < 0.01, ***P < 0.001. (Student’s t test).