Fig. 5.

Expression of progesterone 5β-reductases and cardenolide in shoot cultures of D. lanata after progesterone treatment. a Relative RNA expression levels were calculated by qPCR method by applying the 2^−ΔΔCT method using actin as reference gene. The y axis denotes the normalized relative transcript accumulation of DlP5βR1 and DlP5βR2 genes after progesterone treatment indicated by black arrows. (Mean ± SEM are shown; n = 3). b Quantification of digoxigenin and digitoxigenin in D. lanata WT shoots after progesterone treatment indicated by black arrow. (Mean ± SEM are shown; n = 3)