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. 2021 Jun 19;40(9):1631–1646. doi: 10.1007/s00299-021-02707-3

Fig. 5.

Fig. 5

Expression of progesterone 5β-reductases and cardenolide in shoot cultures of D. lanata after progesterone treatment. a Relative RNA expression levels were calculated by qPCR method by applying the 2−ΔΔCT method using actin as reference gene. The y axis denotes the normalized relative transcript accumulation of DlP5βR1 and DlP5βR2 genes after progesterone treatment indicated by black arrows. (Mean ± SEM are shown; n = 3). b Quantification of digoxigenin and digitoxigenin in D. lanata WT shoots after progesterone treatment indicated by black arrow. (Mean ± SEM are shown; n = 3)