Skip to main content
. Author manuscript; available in PMC: 2022 Aug 18.
Published in final edited form as: Neuron. 2021 Jun 30;109(16):2590–2603.e13. doi: 10.1016/j.neuron.2021.06.005

Figure 3. Inputs and Electrophysiological Properties of AD and AV Thalamus.

Figure 3.

(A) FISH staining in ATN.

(B-D) Mapping brain-wide inputs to AD or AV. RV starters (yellow) in AD (B) or AV (C), average RV-positive cell counts (D) (n = 3 mice for AD, n = 4 mice for AV, normalized starters across groups). PrL (prelimbic cortex), Cg1 (cingulate cortex area 1), Cg2 (cingulate cortex area 2), M2 (secondary motor cortex), S1BF (primary somatosensory cortex barrel field), RSA (retrosplenial agranular cortex), RSG (retrosplenial granular cortex). Dashed line in panel C indicates the border between AD and AV, see also Figures S2E–S2F.

(E-I) RV-GFP labeling (E) of AD neurons (green), recorded neurons (red) (F), after-depolarization potential (ADP) amplitude (G), Ih current-induced sag (H), excitability (I) (22 AD RV+, 17 AD RV−, 18 AV neurons, n = 3 mice).

(J-K) Terminals of ChR2-eYFP injected into PreSub (left) or ChR2-mCherry injected into RSC (right) (J), connectivity between AD, AV, PreSub, and RSC (K).

One-way ANOVA followed by Bonferroni post-hoc test (G-H), and two-way ANOVA with repeated measures followed by Bonferroni post-hoc test (I). For statistical comparisons, ***p < 0.001; NS, not significant.

Data are presented as mean ± SEM.