Fig. 5. Involvement of a PI3K/Akt/GSK-3β pathway in and effect of the TRPV6 function on breast cancer development.
a Representative WB data showing changes in EMT and antiapoptotic markers in MCF-7 (left) and MDA-MB-231 stable cell lines. Statistical analysis of WB data of the expression of indicated markers in MCF-7 (b) and MDA-MB-231 (c) stable cells. d, e Effects of BAPTA-AM (10 μM, 1 h) and PI3K inhibitor LY294002 (50 μM, 1 h) on the EMT and antiapoptosis markers, with DMSO treatment as control. Shown are representative WB data obtained using MCF-7 (d) and MDA-MB-231 (e) stable cells. *p < 0.05; **p < 0.01; ***p < 0.001, from three independent experiments.