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. 2021 Aug 19;4:933. doi: 10.1038/s42003-021-02448-9

Fig. 7. Reduction of systemic formaldehyde rescued motor functions in the mice of hindlimb unloading.

Fig. 7

a The experimental flow. HU: mice of hindlimb unloading; Q10: HU mice intragastrically administered (i.g.) 30-nm coenzyme Q10; RL: HU mice illuminated with 630-nm red light. b The spontaneous chemical reaction between Q10 and formaldehyde. FA formaldehyde. c Metabolism of formaldehyde and coenzyme Q10 in the mitochondria. d Coenzyme Q10 levels quantified by the ECA-fluorescence method (n = 6). e FDH activity detected using an FDH kit (n = 6). FDH formaldehyde dehydrogenase. f Schematic diagram of the effects of and the activation of the Cys97 and Cys100 residues in FDH by 630-nm red light (PDB: 1MC5). Cys: cysteine; Cys111 (green); Cys103 (purple); Cys97 (dusty blue); Cys100 (yellow). g, h Formaldehyde levels quantified by HPLC (n = 6). HPLC high-performance liquid chromatography. i, j Motor functions assessed by accelerating rotarod and beam walking tests, respectively (n = 8). Error bars show the mean ± S.E.M; **p < 0.01; ***p < 0.001.