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. 2021 Aug 19;4:991. doi: 10.1038/s42003-021-02516-0

Fig. 4. RNA-Seq analysis for punC mRNA expression.

Fig. 4

aE. coli MG1655 and the nac mutant were grown in M9 medium in the absence or presence of 10 mM adenine. b mRNA differential expression for the nac mutant and wild-type in E. coli MG1655. Volcano plot comparing the log-fold expression change of genes against the adjusted p-value for the nac mutant and WT MG1655 grown at M9 supplemented with 10 mM adenine. Genes in red dots are differentially expressed. punC (b1660) is the gene with the strongest expression change in addition to hisG (b2019) and hisD (b2020) histidinol dehydrogenase (L-histidine biosynthesis), fimA (b4314), fimI (b4315) and fimC (b4316).