Fig. 3.

Combined loss of meprins delays re-epithelialization. A, Hematoxylin & eosin (H&E) staining of sections from the middle of the wounds 3 or 7 days after wounding or keratin 14 (K14) staining to better visualize the epidermal tongue in 3-day-old wounds. White line indicates the epidermal tongue. Nuclei were counterstained with DAPI (blue). Scale bar = 50 μm for the two top panels and = 100 μm for the bottom panel. B, Bar graph showing quantification of percentage re-epithelialization of 3-day wounds as determined by histological measurements. Individual values for left and right wounds of the same animals (n = 9–18), mean ± S.D. are shown, **P < 0.01 as analyzed by one-way ANOVA with Tukey’s correction. C, Flow cytometry analysis for the abundance of neutrophils and macrophages in healthy 3-day wounds. Values for individual wounds (n = 4–10), mean ± S.D. are shown, *P < 0.05 as analyzed by one-way ANOVA with Tukey’s correction. D, Staining of 3-day wounds for the macrophage marker F4/80. Nuclei were counterstained with DAPI (blue). Scale bar = 50 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)