Figure 3.

Changes in SCFA levels in the feces of rats after different treatments

A. Concentration of acetic acid in feces of rats from different groups before and after treatment. B. Concentration of propionic acid in feces of rats from different groups before and after treatment. C. Concentration of butyric acetic in feces of rats from different groups before and after treatment. D. Spearman’s correlation coefficient of microbial genera abundance and SCFAs was visualized in a hierarchically clustered heatmap. Red indicates a positive correlation and blue a negative correlation. The Kruskal–Wallis test followed by the pairwise Wilcoxon rank sum test was applied to compare different untreated or treated groups. The Wilcoxon rank sum test was used to compare a group before and after treatment. Differences with P < 0.05 (or BH adjusted P < 0.05) were considered to be significant. *, P < 0.05; **, P < 0.01. n = 5–6 samples per group. SCFA, short-chain fatty acid; BH, Benjamini–Hochberg.