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. 2021 Aug 2;12(3):465–473. doi: 10.1016/j.jaim.2021.04.007

Fig. 2.

Fig. 2

Picroside II attenuates lipid accumulation in FFAs loaded cells: The cytotoxic effect of picroside II at 300 μM vs control cells ∗p < 0.05 (A) and Silibinin (positive control) at 300 μM vs control cells ∗p < 0.05 (B). HepG2 cells were pre-treated with picroside II and silibinin at a concentration of 10 μM for 2 h, followed by FFAs - 1000 μM challenge for another 20 h. Microscopic representation of the inhibitory effects of picroside II, and silibinin on FFAs accumulation using ORO stain (C). Quantification of lipid accumulation with ORO colorimetric assay, picroside II vs FFAs – 1000 μM ∗∗p < 0.01 and silibinin vs FFAs −1000 μM ∗p < 0.05 (D). The values are expressed as mean ± SEM, N = 4, n = 3.