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. 2021 Aug 6;11:712348. doi: 10.3389/fonc.2021.712348

Figure 3.

Figure 3

Depletion of ARTN inhibits oncogenic behaviors of CRC cells. (A) Total cell number of DLD1-psilencer and DLD1-siARTN cells under normal culture condition (medium with 10%FBS) or serum deprivation conditions (medium with 1% FBS). (B) BrdU incorporation assay determined S-phase entry of DLD1-psilencer and DLD1-siARTN cells cultured under serum deprivation conditions for 24 hours. (C) Hoechst 33258 staining determined apoptotic nuclei percentages of DLD1-psilencer and DLD1-siARTN cells cultured under serum deprivation condition for 24 hours. (D) Soft agar colony formation of DLD1-psilencer and DLD1-siARTN cells. The colony numbers were counted and presented as relative percentage change. (E) 3D Matrigel growth of DLD1-psilencer and DLD1-siARTN cells. Cell viability was measured by AlamarBlue assay and presented as the relative percentage changes. (F) Transwell migration assay with DLD1-psilencer and DLD1-siARTN cells. (G) Transwell invasion assay with DLD1-psilencer and DLD1-siARTN cells. (H, I), Western blot analysis for the expression of epithelial and mesenchymal cell markers and EMT regulator SNAIL in the stable cell lines with forced expression (H) or depletion (I) of endogenous ARTN. *p < 0.05, **p < 0.01.