Table 5.

Real time-PCR analysis for mRNA expression of CSC markers in DLD1 cells with either forced or depleted expression of ARTN.

Gene	DLD1-ARTN vs. DLD1-vec		DLD1-siARTN vs. DLD1-siVec
	Fold Change	p value	Fold Change	p value
TIMP1	0.84	0.01	1.61	0.001
TIMP3	1	0.031	2.6	0.003
MET	4.45	0.022	0.54	0.001
CD24	0.38	0.001	1.46	0.097
CD44	3.83	0.003	0.28	0.003
CSF1	1.2	0.009	0.42	0.004
KLF4	0.81	0.017	2.1	0.025
NCAM1	1.6	0.006	2.96	0.01
ALDH1	5.47	0.004	0.29	0.021
WNT5A	11.99	0.046	1.88	0.171
WNT5B	2.3	0.068	1.6	0.081
BMI1	0.01	<0.001	0.59	0.001
LIN28A	7.74	0.023	1.3	0.456
SOX2	1.37	0.37	0.52	0.013
POU5F1	1.48	0.001	1.63	0.368
SALL4	4.62	0.002	2.42	0.008

Gene expression is represented in fold change relative to the individual control cells, respectively. Fold change values are representative of three independent biological experiments. To compensate for potential differences between markers, the relative expression was computed, based on the efficiency (E), normalized by three housekeeping genes β-ACTIN, HPRT, and GAPDH.

P values in bold are considered as significant (p < 0.05).