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. 2021 Aug 6;12:713544. doi: 10.3389/fpls.2021.713544

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Copyright © 2021 Liu, Sun, Huang, Li, Zheng, Yuan, Chen, Jan, Zheng, Du and Tu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The interaction between Osj10gBTF3/OSHSP82 and OsPPR676 is different. (A) Y2H assays were performed to test the interaction between OsHSP82 and OsPPR676. The pGADT7-T cells co-transformed with pGBKT7-53 or the pGBKT7-Lam were used as positive or negative controls, respectively. Combinations AD-OsHSP82 with BK empty and combinations BK-OsPPR676 with AD empty were also used as negative controls. SD, Synthetic dextrose. (B) Co-IP assays were performed to test the interaction between Osj10gBTF3/OsHSP82 and OsPPR676 using Arabidopsis protoplasts. FLAG antibody conjugated agarose gel was used to co-precipitate OsPPR676. OsHSP82/Osj10gBTF3 and OsPPR676 were detected by FLAG and His antibody, respectively. Three independent replicates were performed for the experiment. (C) and (D) YFP^N-OsPPR676 was co-transfected with either OsHSP82-YFP^C (C) or Osj10gBTF3-YFP^C (D) into the epidermal cells of tobacco. Chloroplast and mCherry-HDEL (endoplasmic reticulum marker) were used as markers. The intensity profile of the dashed line across the ER (top) or the chloroplast (bottom) in the cells was plotted for the quantification of the fluorescence intensity profiles (right). Scale bar = 10 μm. Negative controls were showed in Supplementary Figure 3.