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. 2021 Aug 6;9:671029. doi: 10.3389/fcell.2021.671029

FIGURE 2.

FIGURE 2

MAR assay and trichrome staining results indicate contrasting effects of Tle4 loss on bone formation and calcification. T4F cre mice of 8-to-9 days old have decreased (A) MAR and (B) BFR rates, measurements of average bone calcification per osteoblast and total bone formation, respectively, while having similar (C) ratio of mineralizing to bone surface ratios. (D) T4F cre mice also had less N.Ob/B.Pm values, an estimate of the number of osteoblasts per area of bone surface. (E) Trichrome staining reveals T4F cre mice have significantly reduced osteoid compared to T4F controls (n = 3 mice per arm; *p < 0.05, Student t-test). (F) Representative image demonstrating decreased bone formation in T4F cre mice vs. T4F mice via MAR double-stain of calcein and demeclocycline.