FIGURE 5.

Tle4 knockdown in ST2D cells cause aberrant levels of Bmp2 and Bmp4 expression in response to Runx2 expression. (A) Twenty-four hours after Runx2 induction, upregulation of Bmp2 in control (CTL) cells. Knockdown of Tle4 represses Bmp2 expression and blocks upregulation with Runx2 induction. (B) Induction of Runx2 also leads to an upregulation of Bmp4 in control cells. This upregulation is not seen in the presence of Tle4 knockdown (n = 3 biologic replicates per arm, technical triplicates; *p < 0.05, **p < 0.01, ***p < 0.001, and Student t-test).