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. 2021 Aug 6;9:705302. doi: 10.3389/fcell.2021.705302

FIGURE 1.

FIGURE 1

Choline kinase alpha expression levels are up-regulated in models of liver regeneration in vivo and in vitro. (A–C) C57B6/L mice were subjected to 2/3 PHx and sacrificed at indicated time points. Chka expression was examined by qPCR (A), western blotting (B), and immunohistochemical staining (C). (D–F) C57B6/L mice were subjected to APAP injection (300 mg/kg) and sacrificed at indicated time points. Chka expression was examined by qPCR (D), Western blotting (E), and immunohistochemical staining (F). (G,H) Primary hepatocytes were isolated from C57B6/L mice and treated with or without HGF. Chka expression levels were examined by qPCR and Western blotting. *p < 0.05.