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. 2021 Aug 6;12:687304. doi: 10.3389/fmicb.2021.687304

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Copyright © 2021 Chu, Wang, Zhang, Li, Wang, Dai and Tang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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DGGE pattern of nested PCR-amplified rRNA gene fragments of fungi from P. tabulaeformis rhizosphere soils (A-C represent rhizosphere soils were sampled at 3, 6, and 9 months after PWN inoculation) of different ECMF and DSE inoculation treatments after PWN inoculation. Lanes labeled Gc, Sb, Av, and Pc in A-C represent P. tabulaeformis seedlings were inoculated with G. cylindrosporus, Suillus bovinus, A. vaginata, and P. chrysanthemicola, respectively; Ck as control group. “+” and “−” represent P. tabulaeformis seedlings were inoculated with PWN and were not inoculated with PWN, respectively. The linear gradient used was from 40 to 65% denaturant. The bands labeled 3S1-3S42 in A, 6S1-6S31 in B, and 9S1-9S18 in C were selected and excised from the DGGE gels and used for cloning and sequencing.