Figure 4.

DGGE pattern of nested PCR-amplified rRNA gene fragments of bacteria from P. tabulaeformis rhizosphere soils (A-C represent rhizosphere soils were sampled at 3, 6, and 9 months after PWN inoculation) of different ECMF and DSE inoculation treatments after PWN inoculation. Lanes labeled Gc, Sb, Av, and Pc in A-C represent P. tabulaeformis seedlings were inoculated with G. cylindrosporus, S. bovines, A. vaginata, and P. chrysanthemicola, respectively; Ck as control group. “+” and “−” represent P. tabulaeformis seedlings were inoculated with PWN and were not inoculated with PWN, respectively. The linear gradient used was from 40 to 65% denaturant. The bands labeled 3B1-3B36 in A, 6B1-6S26 in B, and 9B1-9B29 in C were selected and excised from the DGGE gels and used for cloning and sequencing.