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. 2021 Aug 20;17(8):e9895. doi: 10.15252/msb.20209895

Figure EV1. Scored developmental events and coefficient of variation (CV) analysis.

Figure EV1

  1. Sketches of 12 developmental scores determined to be the most reproducible, in D. melanogaster. Please see Materials and Methods and Movie EV1 for definition of scoring criteria.
  2. Seven additional developmental events in fly embryos that we did not pursue due to poor reproducibility i.e. cut score. The number score code is used in the following CV analysis.
  3. We calculated CVs using preliminary data for every developmental time interval between the 19 scores (described in Materials and Methods) we considered investigating (Dataset EV1). CVs are calculated for each of the 6 different temperatures (n = 3–5 biological replicates per temperature), and the mean CV is then displayed for each interval. CV’s are displayed as percentages. The 11 most reproducible intervals for neighboring scores (diagonal) are shown in green. Intervals shown in red had their associated score (numbers) cut from our investigation.
  4. Sketches of 12 developmental event we investigated in X. laevis determined most reproducible. Please see Materials and Methods and Movie EV2 for definition of scoring criteria.
  5. As (B) but for four additional frog developmental events not included in our final analysis.
  6. As (C) but for intervals calculated from early data, between 16 frog developmental scores considered (described in Material and Methods) averaged over 16 temperatures (n = 2–6 biological replicates per temperature) (Dataset EV2). The 11 most reproducible intervals for neighboring scores (diagonal) are shown in green.