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. 2021 Aug 12;46(4):218. doi: 10.3892/or.2021.8169

Figure 3.

Figure 3.

circNRIP1 acts as a sponge for miR-532-3p in CRC cells. (A) The complementary binding site between miR-532-3p and circNRIP1 is presented. (B) miR-532-3p expression in CRC in The Cancer Genome Atlas database. (C) The relative expression levels of miR-532-3p in paired CRC and adjacent normal tissues were measured by reverse transcription-quantitative PCR (n=72). (D) miR-532-3p expression is negatively associated with the expression of circNRIP1 in CRC tissues. (E) The interaction between miR-532-3p and circNRIP1 was confirmed by a dual-luciferase reporter assay. (F) The expression levels of miR-532-3p in normal and CRC cell lines are presented. (G) Effect of cell transfection of miR-532-3p mimics or its inhibitor on miR-532-3p expression. (H) The effect of cell transfection with si-cNRIP1 on miR-532-3p expression is shown. **P<0.01 and ***P<0.001 vs. NCM460, si-NC group or as indicated. c/circNRIP1, circRNA nuclear receptor-interacting protein 1; CRC, colorectal cancer; si, small interfering RNA; NC, negative control; hsa, Homo sapiens; MUT, mutant; WT, wild-type; miR, microRNA.