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Schematic representation of the procedure used to prepare wrappER-mitochondria (WAM)-enriched fractions
(A) 7 mL of B27 are added into a 50 mL Falcon tube, on top of which 7 mL of post-nuclear liver homogenate are gently layered. The solution is centrifugated in a fixed rotor. The pellet is enriched in WAM.
(B) An image of the pellet enriched in WAM. Note that its appearance depends on the nutritional status of the mouse liver from which it was prepared.
