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. 2021 Aug 16;2(3):100752. doi: 10.1016/j.xpro.2021.100752
REAGENT or RESOURCE SOURCE IDENTIFIER
Chemicals, peptides, and recombinant proteins

EGTA Sigma-Aldrich #E4378
Halt Protease Inhibitor Cocktail (PIC) (100×) Thermo Fisher Scientific #78429
Magnesium chloride hexahydrate Sigma-Aldrich #M9272
Sucrose Thermo Fisher Scientific #BP220-212
Trizma-base Thermo Fisher Scientific #T395
Osmium tetroxide Electron Microscopy Sciences #19152
Uranyl acetate Electron Microscopy Sciences #22400
Propylene oxide Electron Microscopy Sciences #82320
Lead nitrate Electron Microscopy Sciences #17900
Sodium nitrate Electron Microscopy Sciences #21140
Durcupan, single component A Sigma-Aldrich #44611
Durcupan, single component B Sigma-Aldrich #44612
Durcupan, single component C Sigma-Aldrich #44613
Durcupan, single component D Sigma-Aldrich #44614
Glutaraldehyde solution, 25% in H2O Sigma-Aldrich #G5882
Calcium chloride, anhydrous Thermo Fisher Scientific #S25223

Other

Teflon Dounce homogenizer 5 mL Wheaton #358034
30 mL Round-bottomed glass centrifuge tubes Kimble #45500-30
Rotor adapter for 30 mL glass centrifuge tubes Kimble #45550-30
Rotor adapter Sigma-Aldrich #EP022637266-2EA
Glass disposable Pasteur pipettes VWR International #14673-043
Straight operating scissors: sharp/blunt blades Stoelting Co #52136-12P
Sandpaper 240 and 1200 grit N/A N/A
15 mL and 50 mL Polypropylene Falcon tubes N/A N/A
50 mL Pyrex glass beaker N/A N/A
50 mL Plastic beaker Electron Microscopy Sciences #60968
2 mL Plastic transfer pipette SARSTEDT #86.1176.300
Single Slot Copper grids Electron Microscopy Sciences #G2010-Cu

Biological samples

Adult male C57BL/6N mice (8 weeks of age) Charles River Stain code: 027