Chemicals, peptides, and recombinant proteins |
|
EGTA |
Sigma-Aldrich |
#E4378 |
Halt Protease Inhibitor Cocktail (PIC) (100×) |
Thermo Fisher Scientific |
#78429 |
Magnesium chloride hexahydrate |
Sigma-Aldrich |
#M9272 |
Sucrose |
Thermo Fisher Scientific |
#BP220-212 |
Trizma-base |
Thermo Fisher Scientific |
#T395 |
Osmium tetroxide |
Electron Microscopy Sciences |
#19152 |
Uranyl acetate |
Electron Microscopy Sciences |
#22400 |
Propylene oxide |
Electron Microscopy Sciences |
#82320 |
Lead nitrate |
Electron Microscopy Sciences |
#17900 |
Sodium nitrate |
Electron Microscopy Sciences |
#21140 |
Durcupan, single component A |
Sigma-Aldrich |
#44611 |
Durcupan, single component B |
Sigma-Aldrich |
#44612 |
Durcupan, single component C |
Sigma-Aldrich |
#44613 |
Durcupan, single component D |
Sigma-Aldrich |
#44614 |
Glutaraldehyde solution, 25% in H2O |
Sigma-Aldrich |
#G5882 |
Calcium chloride, anhydrous |
Thermo Fisher Scientific |
#S25223 |
|
Other |
|
Teflon Dounce homogenizer 5 mL |
Wheaton |
#358034 |
30 mL Round-bottomed glass centrifuge tubes |
Kimble |
#45500-30 |
Rotor adapter for 30 mL glass centrifuge tubes |
Kimble |
#45550-30 |
Rotor adapter |
Sigma-Aldrich |
#EP022637266-2EA |
Glass disposable Pasteur pipettes |
VWR International |
#14673-043 |
Straight operating scissors: sharp/blunt blades |
Stoelting Co |
#52136-12P |
Sandpaper 240 and 1200 grit |
N/A |
N/A |
15 mL and 50 mL Polypropylene Falcon tubes |
N/A |
N/A |
50 mL Pyrex glass beaker |
N/A |
N/A |
50 mL Plastic beaker |
Electron Microscopy Sciences |
#60968 |
2 mL Plastic transfer pipette |
SARSTEDT |
#86.1176.300 |
Single Slot Copper grids |
Electron Microscopy Sciences |
#G2010-Cu |
|
Biological samples |
|
Adult male C57BL/6N mice (8 weeks of age) |
Charles River |
Stain code: 027 |