Table 2.
Reverse Transcription–Quantitative Polymerase Chain Reaction Primer and Probe Designs for Small Interfering RNA Quantification
| siRNA | Primer | Sequence (5′–3′) |
|---|---|---|
| AD-60519 | Stem–loop | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACACCAGAAAGA |
| Forward qPCR | GCCGCGCTAAGATGAGACA | |
| TaqMan probe | CTGGATACGACACCAGAAA | |
| siRNA2 | Stem–loop | Proprietary |
| Forward qPCR | Proprietary | |
| TaqMan probe | Proprietary | |
| AD-65644 | Stem–loop | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCAGAATGTGA |
| Forward qPCR | GCCGTTGTCGATGACTTT | |
| TaqMan probe | TGGATACGACCAGAATG | |
| AD-64543 | Stem–loop | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAGCACTGTGA |
| Forward qPCR | GCTTGGAGGCCACAGTC | |
| TaqMan probe | TGGATACGACAGCACT | |
| AD-68435 | Stem–loop | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAGTGTGCAATG |
| Forward qPCR | GCCGCGCATATTTGCCTTTC | |
| TaqMan probe | CTGGATACGACAGTGTGC | |
| Universal | Reverse qPCR | GTGCAGGGTCCGAGGT |
qPCR, quantitative polymerase chain reaction.