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4% PFA fixation buffer
| Reagent | Final concentration | Amount |
|---|---|---|
| 32% Paraformaldehyde Solution (EM Grade) | 4% (w/v) | 10 mL |
| PBS | n/a | 70 mL |
| Total | n/a | 80 mL |
It is recommended to prepare fresh PFA buffer on the same day of the experiment and use fresh buffer every time for best outcome.
