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. 2021 Aug 17;2(3):100758. doi: 10.1016/j.xpro.2021.100758

2× cytokine solution (for 60 wells including 10% extra volume)

Reagent Final concentration Amount
X-VIVO 15 n/a ~6.6 mL
Human GM-CSF 2000 IU/mL 4.4 μL
Human IL-4 1000 IU/mL 3.3 μL
Human Flt3-L 100 ng/mL 6.6 μL
Total n/a 6.6 mL