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. 2021 Aug 17;2(3):100758. doi: 10.1016/j.xpro.2021.100758

2× adjuvant solution (for 60 wells including 10% extra volume)

Reagent Final concentration Amount
X-VIVO 15 n/a ~6.6 mL
R848 20 μM 13.2 μL
LPS 200 ng/mL 1.32 μL
IL-1β 20 ng/mL 26.4 μL
Total n/a 6.6 mL