Reagent | Final concentration | Amount |
---|---|---|
RPMI 1640 Medium | n/a | 435 mL |
HEPES (1M) | 10 mM | 5 mL |
Gentamicin (10 mg/mL) | 0.1 mg/mL | 5 mL |
GlutaMAX-I (100×) | 1× | 5 mL |
Human serum | 10% | 50 mL |
Total | n/a | 500 mL |
Reagent | Final concentration | Amount |
---|---|---|
RPMI 1640 Medium | n/a | 435 mL |
HEPES (1M) | 10 mM | 5 mL |
Gentamicin (10 mg/mL) | 0.1 mg/mL | 5 mL |
GlutaMAX-I (100×) | 1× | 5 mL |
Human serum | 10% | 50 mL |
Total | n/a | 500 mL |