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. 2021 Aug 17;2(3):100758. doi: 10.1016/j.xpro.2021.100758

2× re-stimulation solution (for 60 wells including 10% extra volume)

Reagent Final concentration Amount
R10 media n/a ~6.6 mL
Anti-CD28 (0.5 mg/mL) 2 μg/mL 26.4 μL
Anti-CD49d (0.5 mg/mL) 2 μg/mL 26.4 μL
Total n/a 6.6 mL