0.01 M β-mercaptoethanol	Final concentration	Amount
β-mercaptoethanol	10 μM	0.7 μL
ddH2O	n/a	999.3 μL
Total	n/a	1 mL

Mix both nick translation buffer and β-mercaptoethanol dilution by vortexing, and store at −20°C for up to 6 months.