Nucleotide mixture stock	Final concentration	Amount
25 mM dATP	166 μM	4 μL
25 mM dCTP	166 μM	4 μL
25 mM dGTP	166 μM	4 μL
1 mM Dyomics labeled dUTP	166 μM	100 μL
1 M Tris pH 7.4	20 mM	8 μL
ddH2O	n/a	280 μL
Total	n/a	400 μL

Make on ice, mix by vortexing. Aliquot to prevent freeze-thaw cycles, store in opaque tubes to protect from light at −20°C. Store for up to 6 months.