Hybridization buffer	Final concentration	Amount
50% w/v dextran sulfate	15%	300 μL
Deionized formamide, pH 7.0	50%	500 μL
20× SSC	2×	100 μL
10% Tween-20	1%	100 μL
Total	n/a	1 mL

Mix by gentle inversion for at least 18 h to thoroughly mix without adding bubbles. Store at 20°C–22°C. Store for up to 6 months.