| Permeabilization buffer | Final concentration | Amount |
|---|---|---|
| 10% Triton-X 100 | 0.5% | 500 μL |
| Saponin | 0.5% w/v | 50 μg |
| PBS | n/a | QS to 10 mL |
| Total | n/a | 10 mL |
Mix by vortexing. Make fresh on the day of the experiment.
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