Skip to main content
. Author manuscript; available in PMC: 2022 Jun 9.
Published in final edited form as: J Am Chem Soc. 2021 May 31;143(22):8488–8498. doi: 10.1021/jacs.1c03321

Figure 4.

Figure 4.

Summed and normalized area for each mono-phosphorylated and bis-phosphorylated 26xS7K yeast peptide, showing more enhanced CDK7 phosphorylation of the two consensus isomers represented by s40 and s47. Mono-phosphorylated peptides are phosphorylated a second time by CDK7, with an uptick in bis-phosphorylation observed at 240 and 360-minute marks aligning with the decrease in abundances of the consensus mono-phosphorylated peptides. Monitored transitions for the UVPD-PRM method are listed in the Supplementary Transition List. The numbers in purple above the sequence indicate the heptad repeat number along the 26xS7K yeast construct, and subscript numbering indicates residue numbers in every heptad along each tryptic 26xS7K yeast peptide.