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. 2021 Aug 20;21:842. doi: 10.1186/s12879-021-06539-0

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — a Bright-field microscopy with Wright's-Giemsa staining (× 1000). b Phase-contrast microscopy of a sample taken during exercise (wet smear, × 1000). As indicated by the arrows, the 4 characteristic free anterior flagella, nucleus, and axial column of the T. tenax trophozoites were well stained. The undulating membrane is shorter than the long axis of the trophozoite, and accounts for about half of the whole trophozoite body. The slender axial column runs through the trophozoite and extends out of the body from the back, and the axostyle is relatively thick. The nucleus is located in the anterior part of the trophozoite, and has an oval shape with many chromatin granules. c Sputum smear, showing a large number of hyphae from G. capitatum (× 1000)