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. 2021 Aug 19;22:235. doi: 10.1186/s13059-021-02458-0

Fig. 4.

Fig. 4

The web portal interface for Easy-Prime. a Screenshot of the Easy-Prime web portal (based on DASH [33]). Easy-Prime takes a file in vcf or fasta format as input. It searches and optimizes all individual sgRNA-PBS-RTT-ngRNA combinations and visualizes the gRNAs with the highest predicted efficiency for each input variant. b An interactive PE design visualization based on the ProteinPaint genome browser