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. 2021 Aug 19;27:92. doi: 10.1186/s10020-021-00351-x

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Low expression of CB2 was observed in both human and rat degenerative NP tissue. A Preoperative MRI of participants, who were divided into 4 groups (level: I, III, IV, V) according to the Pfirrmann classification. B Safranin O staining of participant NP tissues; scale bar, 1000 μm. C Representative specimens of immunohistochemical CB2 staining in the 4 separate groups (level: I, III, IV, and V) of participant NP tissues; scale bar, 100 μm. D Quantitative analysis of IHC staining of CB2 (*p < 0.05). E Representative MRI showing the signal intensity of IVD in rats. F H&E and safranin O staining, scale bar, 1000 μm. G Representative specimens of paraffin sections with IHC of CB2 in normal and IVDD rats. H Quantitative analysis of CB2 IHC staining (*p < 0.05). (n = 5 Independent experiments)