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. 2021 Aug 20;22:237. doi: 10.1186/s13059-021-02448-2

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Characterization of a megabase-scale PAV, RegionA, in diverse maize inbreds and their wild relatives. a–c Fluorescence in situ hybridization (FISH) of RegionA-specific oligo probes on root tip metaphase chromosomes of B73, Mo17, and their F1 hybrid. RegionA-specific signals (red) were observed on a pair of homologous chromosomes of B73 metaphase cells (a), while absent on Mo17 metaphase chromosomes (b). Only one foci signal can be detected on mitotic metaphase spreads of B73 × Mo17 F1 hybrid (c). Scale bars = 10 μm. d Left: PAV map of RegionA and its flanking regions (2 Mb on each side) in diverse maize inbreds and their wild relatives. Each column represents a bin (dark green for presence bin, light green for absence bin), and each row represents a line (text color of green for Tripsacum; purple for Zea luxurians; yellow for teosintes; red for maize landraces; blue for maize inbreds). Right: The proportion of presence bins in RegionA for each line