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. 2021 Aug 6;11:689626. doi: 10.3389/fonc.2021.689626

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Copyright © 2021 Zhang, Wang, Lu, Su, Wang, Huang, Zhang and Zhu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Detection and validation of risk-related markers of MIBCs. (A) Venn plot shows the overlaps of differentially expressed genes among TCGA and two subsets of GSE48277. (B) Correlation between tumor risk score and expression level of KRT7. TCGA data was used to perform this analysis. Both Pearson correlation coefficient (R) and P-value were calculated. (C) Representative images of KRT7 IHC staining in different risk types and corresponding H&E staining. (D) KRT7 protein expression was significantly decreased in high-risk MIBC specimens compared with low-risk MIBC tissues by IHC. For the details of calculating IHC score, please see Materials and methods.