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. 2021 Aug 6;12:660397. doi: 10.3389/fimmu.2021.660397

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Copyright © 2021 Jiang, Zhang, Zhang, Lu, Sun, Wu, Ding and Huang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A–D) CD3+ T cell, CD4+ T cell, CD8+ T cell, and FOXP3+ regulatory T cell (Treg) infiltration levels in patients with low or high glycoprotein A repetitions predominant (GARP) expression. (E, F) Representative fluorescence-based multiplex immunohistochemistry images. (E) A staining panel was developed to visualize CD3, CD4, CD8, FOXP3, and CK simultaneously on the same tissue slide. (F) A staining panel was developed to visualize CD3, GARP, FOXP3, and CK simultaneously on the same tissue slide. (G, H) Tumor size (T) was associated with changes in CD4+ T cell and FOXP3+ Treg infiltration. Ns, P > 0.05; ***, P < 0.001.