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. 2021 Aug 6;9:715093. doi: 10.3389/fcell.2021.715093

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Copyright © 2021 Helle, Ampuja, Dainis, Antola, Temmes, Tolvanen, Mervaala and Kivelä.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CM characterization. (A) Light-microscope image of hiPS-CMs (scale bar 25 μm), immunofluorescence staining of hiPS-CMs with alpha-actinin, MYL7 and cTnT (scale bar 20 μm). (B) Images at lower magnification illustrate the purity of the CM population (scale bar 50 μm). (C) Light microscope image of hiPS-EC and hiPS-CM co-culture (scale bar 200 μm), immunofluorescence staining hiPS-EC and hiPS-CM co-culture with alpha-actinin and VE-cadherin (scale bar 20 μm).