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. 2021 Aug 6;9:715093. doi: 10.3389/fcell.2021.715093

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Copyright © 2021 Helle, Ampuja, Dainis, Antola, Temmes, Tolvanen, Mervaala and Kivelä.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Co-culture hiPS-CM/EC transcriptomics compared to the human heart, with single cell transcriptome data of three combined samples [HEL24.3 co-culture of hiPS-CMs and hiPS-ECs, HEL47.2 co-culture of hiPS-CMs and hiPS-ECs, and human heart derived cells from a previously published study (Wang et al., 2020)]. (A) UMAP (Uniform Manifold Approximation and Projection) plot of delineated clusters of the combined data. Endothelial cells (EC) and Cardiomyocytes (CM) have been highlighted. (B) Cardiomyocyte, endothelial cell, endocardial cell, fibroblast, pericyte, smooth muscle cell, and cell cycle marker expression in each cluster. (C) The combined UMAP separated according to the three samples.