Skip to main content
. 2021 Aug 6;9:715093. doi: 10.3389/fcell.2021.715093

FIGURE 7.

FIGURE 7

Ligand-target gene analysis using NicheNet (Browaeys et al., 2020). Results are shown for the top 20 ligands. (A) Expression of top 20 ligands in sender cells (hiPS-CM). (B) Ligand–target matrix denoting the regulatory potential between sender (hiPS-CM) ligands and predicted receiver (hiPS-EC) target genes. (C) Ligand-receptor interactions between ligands from hiPS-CMs and receptors expressed from hiPS-ECs. (D) Immunofluorescence staining of ID1 in monoculture hiPS-ECs, monoculture hiPS-CMs, and hiPS-EC/hiPS-CM co-culture (scale bar 20 μm). Predicted cell-cell interactions in co-culture hiPS-EC/hiPS-CM in (E) BMP-pathway, where increased expression of BMP2/7 ligands is seen in hiPS-CMs and BMP4/6 in hiPS-ECs in co-culture as well as an increased expression of the ID-genes in both cell types, (F) Notch-pathway, where the ligand JAG1 is increased in hiPS-ECs and hiPS-CMs and JAG2 and DLL4 in hiPS-ECs, in co-culture resulting in an increased expression of the targets HES1 and HES4 in hiPS-CMs, and HES1, HES4, HEY2 in hiPS-ECs (Data in Supplementary Figure 5). All scRNAseq data presented in the figure consists of combined data from HEL47.2 and HEL24.3 lines.