Skip to main content
. Author manuscript; available in PMC: 2021 Aug 20.
Published in final edited form as: J Med Genet. 2016 Jan 13;53(5):318–329. doi: 10.1136/jmedgenet-2015-103416

Figure 3.

Figure 3

LAMA1-deficient cells exhibit impaired adhesion and migration. (A) Equal numbers of control and patient 2 fibroblasts were plated on wells coated with either bovine serum albumin (BSA), collagen-I (Col-I) or Laminin-111 and allowed to attach. OD values represent amounts of DNA in cells that remain attached to the wells after 90 min of incubation. Bar graph gives mean and SD of five replicates from three independent experiments; *p<0.05; **p<0.01 (Mann–Whitney test). (B) Equal numbers of control and patient 2 fibroblasts were seeded on culture wells and allowed to form a monolayer and grow to confluence, after which a 0.9 mm scratch was made. ‘Wound’ closure was monitored by imaging cells stained with Calcein AM after 12, 24, 36, 48 and 60 h. Percentage closure and migration rates were calculated from six experiments. Scale bar represents 100 μm. Bar graph gives mean and SD of three replicates from three independent experiments; *p<0.05; **p<0.01 (Mann–Whitney test).