TABLE 1.

The main components of the protein binding reaction of the prepared RNA.

Reagent	Volume of each component (per 100 μ L) M l	Range
10X Protein-RNA Binding Buffer	10	5–20 μL
50% glycerin	30	0–50 μL
Lysis solution (protein concentration >2 mg/ml)	20–30	200 μg or more
Nuclease-free ultrapure water	Up to 100	To 100 μL
Total capacity	100