Fig 1. 5’ RACE-PCR immunoglobulin heavy (IgV_H) and light (IgV_L) chain products for each of the 4 hybridoma cell lines.

A. Illustration of the 5’ RACE-PCR amplicons of IgV_H and IgV_L used in this study. The 5’ RACE Universal Primer (5’ RACE UPM) and antisense, 3’ isotype-specific primer (3’ ISP) were marked. B. Agarose gel electrophoresis of the 5’ RACE-PCR amplicons of IgV_H and IgV_L for the 4 hybridoma cell lines. The amplicons were separated by 1.5% low melting agarose electrophoresis with ethidium bromide and were excised, extracted, and column purified. The corresponding amplicons of IgV_H or IgV_L were used either for generating an NGS library for MiSeq Illumina sequencing or for cloning into a TOPO-TA vector, followed by Sanger sequencing.