. 2021 Aug 20;16(8):e0256079. doi: 10.1371/journal.pone.0256079

Table 2. IgV sequences identified by NGS/bioinformatics and Sanger sequencing methods.

Items	Ig Subtypes	transcript tpm^*	transcript read count	transcript avg coverage	TOPO-Sanger finds	IgV validated by recombinant ab: antigen-binding assay
SodA^NRC-13810	heavy, IgG1	963725.1311	88897.61877	10633.68646	Yes	True
	light, Igκ-1	39232.47749	3635.001071	883.7117677	Yes	True
	light, Igκ-2	304067.109	21428.2634	6339.72289	No	True
	light, Igκ-3	38357.25488	2533.438146	783.5375709	No	False No/little Secretion
	light, Igκ-4	13688.0569	849.329898	273.9773865	No	False No/little Secretion
PstS1^NRC-2410	heavy, IgG1	968826.8725	40043.93523	9073.399221	No	True
	light, Igκ-DIV	843113.5427	20217.92773	6151.499309	Yes	True
	light, Igκ-S26	3807.603	74.34237	25.69437	No	False
KatG^NRC-49680	heavy, IgM	610640.4988	17956.89359	4519.352413	No	True
	heavy, IgM_KVS	378156.7621	11071.18364	2795.753444	Yes	False No/little Secretion
	light, Igκ	453166.48	9730.178	2884.44	Yes	True
GroES^NRC-2894	heavy, IgG2a	974745.1045	6680.858709	1397.669186	Yes	True
GroES^NRC-2894	light, Igκ	47876.02964	245.414241	74.97380071	Yes	True

*: transcripts per million transcripts.