Fig 1. Cohort 1 and Cohort 2 and Cohort 1 hyper-immunized: Flow diagram of immunizations, CHMI and PBMC samples.

Panel A: Cohort 1 received four monthly immunizations (1, 2, 3, 4) and a fifth immunization (5) 33 days later; 11 subjects received their first CHMI (CHMI#1) 22–24 days post-5^th; four protected subjects from Cohort 1 were hyper-immunized and received three monthly boosting immunizations (B1, B2, B3) starting 329 days post-1^st, and three subjects received CHMI#2 on days 472 post-1^st. Six/11 (55%) immunized subjects were protected against CHMI#1, and three/three (100%) hyperimmunized subjects were protected against CHMI#2. Panel B: Cohort 2 followed the same schedule as Cohort 1, and nine/10 (90%) immunized subjects were protected against CHMI. PBMC were taken at times indicated (Green asterisk) that were just prior to each immunization and CHMI, and 38 to 44 days post-CHMI, except an additional sample was taken at 7 days post-1^st only in Cohort 1.